Anticancer and Antioxidant Properties of Ag NPs Coated with BSA NPs

Document Type: Full article

Authors

1 - Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran

2 - Faculty of New Science and Technology, University of Tehran, Iran

3 Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran

Abstract

"> There has been considerable interest in developing albumin nanoparticles as drug delivery devices. Albumin is an important endogenous antioxidant due to its potential of acting as reactive oxygen species scavenger. On the other hand, toxicity of silver nanoparticles had been demonstrated on cancer cell lines. In the present study, Ag NPs coated with BSA NPs were synthesized by silver nanoparticles which were coated with bovine serum albumin (BSA) via desolvation technique. The Ag NPs coated with BSA NPs formation was confirmed by UV-Vis spectroscopy and #_>ADF;;==@ Dynamic Light Scattering (DLS). Human breast cancer cells (MCF7 cells) were then cultured in the presence of the nanoparticles to evaluate the cytotoxicity of Ag NPs coated with BSA NPs by the MTT colorimetric technique. The antioxidant activities ofAg NPs coated with BSA NPs were evaluated in terms of their inhibition of autoxidation rate of pyrogallol as superoxide. The effect ofAg NPs coated with BSA NPs on MCF7 exhibit a dose-dependent toxicity for the cell tested and the viability of MCF-7 decreased to 50% (LD50) at the concentration of5 Ïg/mL. The IC50 value ofantioxidant activities ofAg NPs coated with BSA NPs were 8 µg/mL which demonstrated that Ag NPs coated with BSA NPs were good superoxide scavengers. In conclusion, our data show that Ag NPs coated with BSA NPs had antioxidant and anticancer activities in MCF-7 cells.

Keywords


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