@article { author = {Nikpour, M. and Pazouki, M.}, title = {Lipase Immobilized into Novel GPTMS: TMOS Derived Sol-Gels and Its Application for Biodiesel Production from Waste Oil}, journal = {Iranian Journal of Chemical Engineering(IJChE)}, volume = {13}, number = {1}, pages = {32-46}, year = {2016}, publisher = {Iranian Association of Chemical Engineers(IAChE)}, issn = {1735-5397}, eissn = {2008-2355}, doi = {}, abstract = {In this essay, lipase from Burkholderia cepacia was immobilized into 3-glycidoxypropyltrimethoxysilane (GPTMS) and tetramethoxysilane (TMOS) derived sol-gels. GPTMS:TMOS molar ratio of 1:3 was found to yield the best result. The morphological characteristics were investigated based on SEM and BET analysis. Sample mean pore diameter was 39.1 nm, it had a specific surface area of 60 m2/g prior to enzyme addition which decreased to 7.49 m2/g after immobilization. The enzyme activity was assessed through transesterification of waste cooking oil in the presence of ethanol with optimal conditions of: 40 ᵒC, 15 % immobilized lipase, 9:1 alcohol to oil molar ratio in 24 h of reaction which resulted to 91.70 % biodiesel production. In six-hour reaction time, 86.87 % biodiesel was obtained which is much shorter than conventional enzymatic transesterification which is 72 hours. Ethyl esters were characterized by determining their viscosity, density, and flash point based on ASTM D 6751-07b standards.}, keywords = {Immobilization,Lipase,Sol-Gel,Biodiesel,Enzymatic Transesterification}, url = {https://www.ijche.com/article_15375.html}, eprint = {https://www.ijche.com/article_15375_ac278a44649b4945a490b30e900ce1ab.pdf} }