%0 Journal Article %T Lipase Immobilized into Novel GPTMS: TMOS Derived Sol-Gels and Its Application for Biodiesel Production from Waste Oil %J Iranian Journal of Chemical Engineering(IJChE) %I Iranian Association of Chemical Engineers(IAChE) %Z 1735-5397 %A Nikpour, M. %A Pazouki, M. %D 2016 %\ 01/01/2016 %V 13 %N 1 %P 32-46 %! Lipase Immobilized into Novel GPTMS: TMOS Derived Sol-Gels and Its Application for Biodiesel Production from Waste Oil %K Immobilization %K Lipase %K Sol-Gel %K Biodiesel %K Enzymatic Transesterification %R %X In this essay, lipase from Burkholderia cepacia was immobilized into 3-glycidoxypropyltrimethoxysilane (GPTMS) and tetramethoxysilane (TMOS) derived sol-gels. GPTMS:TMOS molar ratio of 1:3 was found to yield the best result. The morphological characteristics were investigated based on SEM and BET analysis. Sample mean pore diameter was 39.1 nm, it had a specific surface area of 60 m2/g prior to enzyme addition which decreased to 7.49 m2/g after immobilization. The enzyme activity was assessed through transesterification of waste cooking oil in the presence of ethanol with optimal conditions of: 40 ᵒC, 15 % immobilized lipase, 9:1 alcohol to oil molar ratio in 24 h of reaction which resulted to 91.70 % biodiesel production. In six-hour reaction time, 86.87 % biodiesel was obtained which is much shorter than conventional enzymatic transesterification which is 72 hours. Ethyl esters were characterized by determining their viscosity, density, and flash point based on ASTM D 6751-07b standards. %U https://www.ijche.com/article_15375_ac278a44649b4945a490b30e900ce1ab.pdf