Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran
Abstract
Plants and/or plant food wastes have been given much less attention or even disregarded. In some instances, however, oxidative enzymes from residual plant tissues have been shown to effectively degrade recalcitrant pollutants. Soybean seed coat peroxidase (SBP) is an inexpensive oxidoreductive enzyme and could be potentially used to oxidize/polymerize various organic pollutants of the industrial and petrochemical wastes. The catalytic properties of SBP are retained under a wide range of pH and at elevated temperatures. In the present study, the biocatalytic properties of SBP were estimated. The enzyme exhibited the highest activity and stability at pH 6.0 and retained over 75% of the maximum activity for 12 hours. The activity of SBP was found to be 2.5 times higher at an elevated temperature of 65°C compared to the activity at room temperature. The activity is retained over 95% for 30 min at 75°C. The pH and temperature of the reaction mixture showed significant influence on SBP activity. SBP is fairly active in organic solvents and exhibited the optimal activity in the presence of 20% (v/v) acetone. Increasing the organic solvent content resulted in a reduction in SBP activity.
Ghaemmaghami,F. , Alemzadeh,I. and Motamed,S. (2010). Seed Coat Soybean Peroxidase: Extraction and Biocatalytic Properties Determination. Iranian Journal of Chemical Engineering (IJChE), 7(2), 28-38.
MLA
Ghaemmaghami,F. , , Alemzadeh,I. , and Motamed,S. . "Seed Coat Soybean Peroxidase: Extraction and Biocatalytic Properties Determination", Iranian Journal of Chemical Engineering (IJChE), 7, 2, 2010, 28-38.
HARVARD
Ghaemmaghami F., Alemzadeh I., Motamed S. (2010). 'Seed Coat Soybean Peroxidase: Extraction and Biocatalytic Properties Determination', Iranian Journal of Chemical Engineering (IJChE), 7(2), pp. 28-38.
CHICAGO
F. Ghaemmaghami, I. Alemzadeh and S. Motamed, "Seed Coat Soybean Peroxidase: Extraction and Biocatalytic Properties Determination," Iranian Journal of Chemical Engineering (IJChE), 7 2 (2010): 28-38,
VANCOUVER
Ghaemmaghami F., Alemzadeh I., Motamed S. Seed Coat Soybean Peroxidase: Extraction and Biocatalytic Properties Determination. IJChE, 2010; 7(2): 28-38.
