Document Type : Full article

Authors

Energy Department, Materials and Energy Research Center, Meshkin Dasht, Karaj, IR Iran

Abstract

"> Long-chain alkanes are a major constituent of crude oils and their conversions into other compounds are of interest depending on the specific application. Here, five native microbial consortia obtained from petroleum polluted sites were examined for biological conversion of n-octadecane as a representative of long chain alkanes. The experiments were implemented in 250 mL flasks containing 0.5 g n-octadecane in 40 mL culture media kept on a shaker at 160 rpm and 30C for one week. A pure culture of Psedumonas putida was inoculated at the same condition for comparison. Amongst the consortia, ABN52 imposed more obvious changes on n-octadecane. The GC-MS analysis of daily samples showed the appearance of lighter branched compounds at the first and second days of incubation but disappeared in the following days. At the end of incubation time up to 20 (w/w%) of the initial substrate was turned into polyhydroxyalkanoates (PHAs). It also suggested higher activity of the consortia compared to the pure culture of Psedumonas putida. Keywords: Bioconversion, Aalkanes, Polyhydroxyalkanoate, PHA, Pseudomonas Putida

Keywords

Main Subjects

[1]        Vazquez-Duhalt, R. and Quintero-Ramirez, R., "Studies in surface science and catalysis, Petroleum Biotechnology Developments and Perspectives", Elsevier, 151, 29 (2004).
[2]        Das, N. and Chandran, P., "Microbial Degradation of Petroleum Hydrocarbon Contaminants, an Overview", Biotechnol. Res. Int., 2011 (2011).
[3]        Yanto, D. H. Y. and Tachibana, S., "Biodegradation of petroleum hydrocarbons by a newly isolated Pestalotiopsis sp. NG007", Int. Biodet. Biodeg., 85, 438 (2013).
[4]        Labinger, J. A. and Bercaw, J. E., "Understanding and exploiting C-H bond activation", Nature, 417, 507 (2002).
[5]        Tapilatu, Y., Acquaviva, M., Guigue, C., Miralles, G., Bertrand, J. C. and Cuny, P.,"Isolation of alkane-degrading bacteria from deep-sea Mediterranean sediments",Lett. App. Microbiol., 50 (2), 234 (2010).
[6]        Roj, F., "Minireview Degradation of alkanes by bacteria", Environ. Microbiol., 11 (10), 2477 (2009).
[7]        Das, K. and Mukherjee, A. K., "Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India", Biores. Technol., 98, 1339 (2007).
[8]        Alzaga, R., Montuori, P., Ortiz, L., Bayona, J. M. and Albaige´s, J., "Fast solid-phase extraction-gas chromatography-mass spectrometry procedure for oil fingerprinting - Application to the Prestige oil spill", J. Chromatography A, 1025, 133 (2004).
[9]        Ta-Chen, L., Chang, J. Sh. and Young, C. C., "Exopolysaccharides produced by Gordonia alkanivorans enhance bacterial degradation activity for diesel", Biotechnol. Lett., 30, 1201 (2008).
[10]      Van Beilen, J. B., Funhoff, E. G., Van Loon, A., Just, A., Kaysser, L., Bouza, M., Holtackers, R., Röthlisberger, M., Li, Z. and Witholt, B., "Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases", Appl. Environ. Microbiol., 72, 59 (2006).
[11]      Hassanshahian, M., Ahmadinejad, M., Tebyanian, H. and Kariminik, A., "Isolation and characterization of alkane degrading bacteria from petroleum reservoir waste water in Iran (Kerman and Tehran provenances)", Marine. Pollution. Bulletin., 73, 300 (2013).
[12]      Balba, M. T., Al-Awadhi, N. and Al-Daher, R., "Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation", J. Microbiol. Methods., 32, 155 (1998).
[13]      Bajpai, U., Kuhad, R. C. and Khanna, S., "Mineralization of [C 14] octadecane by Acinetobacter calcoaceticus S19", J. Microbiol., 44, 681 (1998).
[14]      Lal, B. and Khanna, S., "Mineralization of [C14] octacosane by Acinetobacter calcoaceticus S30", J. Microbiol., 42, 1225 (1996).
[15]      Ghazali, M. F., Zaliha, N. R., Abdul, R. N., Salleh, A. B. and Basri, M., "Biodegradation of hydrocarbons in soil by microbial consortium", Int. Biodet. Biodegr., 54,61 (2004).
[16]      Gerdes, B., Brinkmeyer, R., Dieckmann, G. and Helmke, E., "Influence of crude oil on changes of bacterial communities in Arctic sea-ice", FEMS Microbiol. Ecology., 53, 129 (2005).
[17]      Ghollami, M., Roayaei, M., Ghavipanjeh, F. and Rasekh, B., "Bioconversion of Heavy Hydrocarbon Cuts Containing High Amounts of Resins by Microbial Consorsia", Pet. Environ. Biotechnol., 4, 139 (2013).
[18]      Varjani, S. J. and Upasani, V. N., "Comparative Studies on Bacterial Consortia for Hydrocarbon Degradation", Int. J. Innov. Res. Sci., Eng. Technol., 2 (10), 5377 (2013)
[19]      Rambeloarisoa, E., Rontani, J. F., Giusti, G., Duvnjak, Z. and Bertrand, J. C., "Degradation of crude oil by a mixed population of bacteria isolated from sea-surface foams", Mar. Biol., 83, 69 (1984).
[20]      Bushnell, L. D. and Hass, H. F., "The utilization of certain hydrocarbons by microorganisms", J. Bacteriol., 41, 653 (1941).
[21]      Hong, K., Sun, Q., Tian, W. D., Chen, G. Q. and Huang, W., "A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier-transform infrared spectroscopy", Appl. Microbiol. Biotechnol., 51, 523 (1999).
[22]      Arcos-Hernandez, M., Gurieff, N., Pratt, S., Magnusson, P., Werker, A. and Vargas Lant, P., "Rapid quantification of intracellulal PHA using infrared spectroscopy: An application in mixed cultures", Biotechnol., 150, 372 (2010).
[23]      Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Eggink, G. and Witholt, B., "Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-I-3-Hydroxyalkanoates and Poly-1-3-Hydroxyalkanoates", Appl. Environ. Microbiol., 54 (12), 2924 (1988).
[24]      Odian, G., Principles-of-Polymerization, 4rd ed., Wiley J & Sons, New Jersey, USA, p. 40 (2004).
[25]      Janarthanan, O. M., Yu, Y., Laycock, B., Werker, A. and Pratt, S., "Fractionation of microbial populations in a PHA accumulating mixed culture and associated PHA content and composition", Int. J. Biol. Macromol., 71, 53 (2014).
 [26]     Laycock, B., Halley, P., Pratt, S., Werker, A. and Lant, P., "The chemomechanical properties of microbial polyhydroxyalkanoates", Prog. Poly. Sci., 39,397 (2014).
[27]      Hori, K., Abe, M. and Unno, H., "Production of triacylglycerol and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by the toluene-degrading bacterium Rhodococcus aetherivorans IAR1", J. Biosci. Bioengin., 108, 319 (2009).
[28]      Santhanam, A. and Sasidharan, S., "Microbial production of polyhydroxy alkanotes (PHA) from Alvaligenes spp. and Pseudomonas oleovorans using different carbon sources", African J. Biotechnol., 9, 3144 (2010).