Document Type : Research note


Antioxidants have an important role in control and prevention of dangerous diseases like cancers, but instability and high solubility of the antioxidants are major challenges of pharmaceutical researchers. Thus, using a suitable carrier for an antioxidant can enhance the antioxidant stability and protect it from reacting with the other existing molecules in the blood circulation. Mesoporous silica nanoparticles (MSNs) have been widely used as a carrier for therapeutic applications because of their suitable biological properties. This study attempts to improve the surface properties and increase antioxidant loading by functionaliztion of MSNs with 3-aminopropyltriethoxysilane (AP-MSNs) via post- synthesis grafting method. Synthesized nanoparticles were characterized by Scanning electron microscopy (SEM), Zetasizer and Fourier transform infrared spectroscopy (FTIR). Gallic acid (GA) was loaded into AP-MSNs. To optimize GA loading capacity, two effective parameters: GA concentration and embedding time were investigated. So different concentrations of GA in EtOH (1-50 mg/mL) were prepared and sampling was done in 24 and 48 h. Results showed that the best GA loading capacity was obtained at a concentration of 40 mg/mL in 48 h. The maximum GA loading capacity and entrapment efficiency were obtained 46 and 20%, respectively, determined by spectrophotometry and high-performance liquid chromatography (HPLC) analysis.


Main Subjects

[1]Kaparissides, C. Alexandridou, S. Kotti,   K. and Chaitidou, S.,"Recent Advances in      Novel Drug Delivery Systems", Nanosci. Nanotech. J., 2 (1), 1 (2011).
[2] Rashidi, L. Vasheghani-Farahani, E. Rostami, K. Gangi, F. and Fallahour, M., "Mesoporous Silica Nanoparticles as a Nanocarrier for Delivery of Vitamin C", Iran. J. Biotech., 11 (4), 209 (2013).
[3] Chunga, T. H. Wub, S. H. Yao, M. Lu, C. W. Lin, Y. S. Hung, Y. Mou, Ch. Y. Chena, Y. C. and Huang, D. M., "The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells", Biomaterials, 28 (19), 2959 (2007).
[4] Xu, J. H. Gao, F. P. Li, L. L. Ma, H. L. Fan, Y. S. Liu, W. Guo, S. S. Zhao, X. Z. and Wang, H., "Gelatin–mesoporous silica nanoparticles as matrix metalloproteinases-degradable drug delivery systemsin in vivo", Micropor. Mesopor. Mat., 182 (2), 165 (2013).
[5] Li, X. Tang, T. Zhou, Y. Zhang, Y. and Sun, Y., "Applicability of enzyme-responsive mesoporous silica supports capped with bridged silsesquioxane for colon-specific drug delivery", Micropor. Mesopor. Mat., 184 (143), 83 (2014).
[6] Wang, X. Miao, J. Xia, Q. Yang, K. Huang, X. Zhao, W. and Shen, J., "A high-sensitivity immunosensor for detection of tumor marker based on functionalized mesoporous silica nanoparticles", Electrochimica Acta, 112 (99), 473 (2013).
[7] Tzankov, B. Yoncheva, K. Popova, M. Szegedi, A. Momekov, G. Mihály, J. and Lambov, N., "Indometacin loading and in vitro release properties from novel carbopol coated spherical mesoporous silica nanoparticles", Micropor. Mesopor. Mat., 171, 131 (2013).
[8] Hu. X, Wang. Y and Peng. B, "Chitosan-Capped Mesoporous Silica Nanoparticles as pH-Responsive Nanocarriers for Controlled Drug Release", An Asia. J., 9, 319 (2013).
[9] Kamarudin, N. H. N. Jali, A. A. Triwahyono, S. Salleh, N. F. M. Karim, A. H. Mukti, R. R. Hameed, B. H. and Ahmad, A., "Role of 3-aminopropyltriethoxysilane in the preparation of mesoporous silica nanoparticles for ibuprofen delivery: Effect on physicochemical properties", Micropor. Mesopor. Mat, 180, 235 (2013).
[10]    Cho, Y. Kim, S. K. Ahn, CH. B. and Ja, Y., "Preparation, characterization, and antioxidant properties of gallic acid-grafted-chitosans", Carbohydr. Polymer, 83, 1617  (2011).
[11]    Cho, M. Lee, H. S. Kang, I. J. Won, M. H. and You, S. G., "Antioxidant properties of extract and fractions from Enteromorpha prolifera a type of green seaweed", Food Chem. 127, 999 (2011).
[12]    Pasanphan, W. and Chirachanchai, S., "Conjugation of gallic acid onto chitosan: An approach for green and water-based antioxidant", Carbohydrate Polymer., 72, 169 (2008).
[13]    You, B. Y. Moon, M. J. Hwan Han. Y. and Park, W. H., "Gallic acid inhibits the growth of HeLa cervical cancer cells via apoptosis and/or necrosis", Food Chem. Toxicol., 48, 1334 (2010).
[14]    Sharma, A. Gautam, S. P. and Gupta, A. K., "Surface modified dendrimers: Synthesis and characterization for cancer targeted drug delivery", Bioorg. Med. Chem., 19, 3341 (2011).
[15]    Rashidi, L. Vasheghani-Farahani, E. Rostami, K. Ganji, F. and Fallahour, M. "Mesoporous silica nanoparticles with different pore sizes for delivery of pH- sensitive Gallic acid",  Asia Pac. Chem. Eng., 9 (6), 845 (2014).
[16]    Zhang, Y. Zhi, Z. Jiang, T. Zhang, J. Wang, Z. and Wang, S., "Spherical mesoporous silica nanoparticles for loading and release of the poorly water-soluble drug telmisartan", J. Control. Release., 145 (3), 257 (2010).
[17]    Rashidi, L. Vasheghani-Farahani, E. Rostami, K. Gangi, F. and Fallahour, M.," A cellular uptake and cytotoxicity properties study of gallic acid-loaded mesoporous silica nanoparticles on Caco-2 cells", J.Nanopart. Res., 16, 221 (2014).