Document Type : Regular Article
Ahar Branch, Islamic Azad University
Department of Medical Nanotechnologies, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
In recent years, the development of nanoparticles has received much attention in the controlled drug release and biomedicine fields. This research aims to develop new methods for the physical modification of Fe3O4 superparamagnetic nanoparticles with polymers through the physical retention. In this study, first, the degradable polycaprolactone-ethylene glycol copolymer and magnetic nanoparticles were synthesized. The anticancer drug doxorubicin was prepared using a dual-emulsion (w/o/w) copolymer containing magnetic iron nanoparticles. FT-IR, NMR, XRD, VSM, and, SEM analyzes were used to characterize copolymers and magnetic nanoparticles with drug-containing copolymer coatings. The results showed that nanoparticles had superparamagnetic properties and their particle size was between 70-150 nm. The drug encapsulation efficiency was about 96 %. The influence of pH and temperature on the drug release curve was investigated. The drug release was 31 % and 26 % after 144 hours in pH = 5.8 and 7.4 respectively. Since the extracellular fluid of the tumor is acidic, the rate of the drug release in these media will be better than the same in other cells. The kinetics of the drug release was also studied based on zero-order, first-order, Higuchi and Korsmeyer-Peppas models. Among the kinetic models, Higuchi was found to be the best model based on the correlation coefficient. The performance of the drug-loaded magnetic-copolymer nanoparticles with that of other similar studies was compared. The results revealed that the magnetic PCL-PEG copolymer with pH-sensitive properties can be used as an effective carrier for anticancer drugs delivery.
- Kopecek, J., “Smart and genetically engineered biomaterials and drug delivery systems”, J. Pharm. Sci., 20 (1), 1 (2003).
- Fana, Q. L., Neohb, K. G., Kangb, E. T., Shuter, B. and Wang, S. C., “Solvent-free atom transfer radical polymerization for the preparation of poly(poly(ethyleneglycol) monomethacrylate)-grafted Fe3O4 nanoparticles: Synthesis, characterization and cellular uptake”, Biomaterials, 28 (36), 5426 (2007).
- Khaledian, M., Nourbakhsh, M. S., Saber, R., Hashemzadeh, H. and Darvishi, M. H., “Preparation and evaluation of doxorubicin-loaded PLA–PEG–FA copolymer containing superparamagnetic iron oxide nanoparticles (SPIONs) for cancer treatment: Combination therapy with hyperthermia and chemotherapy”, J. Nanomed., 15, 6167 (2020).
- Byrne, J. D., Betancourt, T. and Brannon-Peppas, L., “Active targeting schemes for nanoparticle systems in cancer therapeutics”, Drug Delivery Rev., 60 (15), 1615 (2008).
- Peppas, L. B. and Blanchette, J. O., “Nanoparticle and targeted systems for cancer therapy”, Drug Delivery Rev., 56 (11), 1649 (2004).
- Ebrahimi, E., Khandaghi, A. A., Valipour, F., Babaie, S., Asghari, F., Motaali, S., Abbasi, E., Akbarzadeh, A. and Davaran, S., “In vitro study and characterization of doxorubicin-loaded magnetic nanoparticles modified with biodegradable copolymers”, Cells Nanomed. Biotechnol., 44 (2), 550 (2014).
- Asadi, N., Annabi, N., Mostafavi, E., Anzabi, M., Khalilov, R., Saghfi, S., Mehrizadeh, M. and Akbarzadeh, A., “Synthesis, characterization and in vitro evaluation of magnetic nanoparticles modified with PCL–PEG–PCL for controlled delivery of 5FU”, Cells Nanomed. Biotechnol., 46 (1), 938 (2018).
- McNeeley, K. M., Karathanasis, E. Annapragada, A. V. and Bellamkonda, R. V., “Masking and triggered unmasking of targeting ligands on nanocarriers to improve drug delivery to brain tumors”, Biomaterials., 30 (23-24), 3986 (2009).
- Banerjee, D. and Sengupta, S., “Progress in molecular biology and translational science”, USA Elsevier Inc, 104, 489 (2011).
- Eatemadi, A., Darabi, M., Afraidooni, L., Zarghami, N., Daraee, H., Eskandari, L., Mellatyar, H. and Akbarzadeh, A., “Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines”, Artif. Cells Nanomed. Biotechnol., 44 (3), 1008 (2016).
- Nikzamir, N., Khojasteh, H., Nobakht Vakili, M., Azimi, C. and Ghanbari, , “Preparation of degeredable polyprolactone polymer (PCL)/magnetic nanocomposite for drug delivery systems against anticancer compounds”, J. Nanostruct., 11 (3), 456 (2021).
- Jahangiri, S. and Akbarzadeh, A., “Preparation and in vitro evaluation of methotrexate-loaded magnetic nanoparticles modified with biocompatible copolymers”, Cells Nanomed. Biotechnol., 44 (7), 1733 (2016).
- Singha, G. and Rajeswari, M. R., “Preferential binding of anti-cancer drug adriamycin to the Sp1 binding site in c-met promoter region: A spectroscopic and molecular modeling study”, Mol. Struct., 920 (1), 208 (2009).
- Vaezifar, S. and Molaei, M., “Preparation and characterization of drug-delivery system of chitosan nanoparticles containing doxorubicin for use in the treatment of breast cancer”, J. Isfahan Med. Sch., 37 (541), 1047 (2019).
- Yong, Y., Bai, Y., Li, Y., Lin, L. and Cui, Y., “Preparation and application of polymer-grafted magnetic nanoparticles for lipase immobilization”, Magn. Magn. Mater., 320 (19), 2350 (2008).
- Lee, S. J., Jeong, J. R., Shin, S. C., Kim, J. C., Chang, Y. H., Chang, Y. M. and Kim, J. D., “Nanoparticles of magnetic ferric oxides encapsulated with poly (D, L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent”, Magn. Magn. Mater., 272-276, 2432 (2004).
- Butoescu, N., Seemayer, C. A., Foti, M., Jordan, O. and Doelker, E., “Dexamethasone-containing PLGA superparamagnetic microparticles as carriers for the local treatment of arthritis”, Biomaterials, 30 (9), 1772 (2009).
- Dave, V., Yadav, R. B., Kushwaha, K., Yadav, S., Sharma, S. and Agrawal, U., “Lipid-polymer hybrid nanoparticles: Development & statistical optimization of norfloxacin for topical drug delivery system”, Mater., 2, 269 (2017).
- Grainger, D. W., “Controlled-release and local delivery of therapeutic antibodies”, Expert Opin. Biol. Ther., 4 (7), 1029 (2004).
- Zhang, L., Chen, Z., Wang, H., Wu, S., Zhao, K., Sun, H., Kong, D., Wang, C., Lenga, X. and Zhu, D., “Preparation and evaluation of PCL–PEG–PCL polymeric nanoparticles for doxorubicin delivery against breast cancer”, RSC Adv., 60 (6), 54727 (2016).
- Liang, Y., Fu, X., Du, C., Xia, H., Lai, Y. and Sun, Y., “Enzyme/pH-triggered anticancer drug delivery of chondroitin sulfate modified doxorubicin nanocrystal”, Cells Nanomed. Biotechnol., 48 (1), 1114 (2020).
- Xu, P., Zuo, H., Chen, B., Wang, R., Ahmed, A., Hu, Y. and Ouyang, J., “Doxorubicin-loaded platelets as a smart drug delivery system: An improved therapy for lymphoma”, Rep., 7, 42632 (2017).
- Haghiralsadat, F., Amoabediny, G., Helder, M. N., Naderinezhad, S., Sheikhha, M. H., Forouzanfar, T. and Zandieh-Doulabi, B., “A comprehensive mathematical model of drug release kinetics from liposomes, derived from optimization studies of cationic pegylated liposomal doxorubicin formulations for drug-gene delivery”, Cells Nanomed. Biotechnol., 46 (1), 169 (2018).