Document Type : Regular Article

Authors

1 Assistant professor @ Shahid Chamran University of Ahvaz

2 technical assistant@ Shahrekord University of Applied Science, Nahangol Branch, Boroujen, Iran

Abstract

The chemical interesterification (CIE) process is a promising approach to modifying and improving oils and fat structure. In this study, CIE of fully hydrogenated soybean oil (FHSO) and sunflower oil (SFO) was performed. Different initial blends with various mass ratios of 20-45% FHSO (coded as S1, S2, S3, and S4) were converted to interesterified samples (Si-1, Si-2, Si-3, and Si-4, peer-to-peer). The interesterified samples (60% content) were used in different margarine formulas with 40% palmolein PO (M1, M2, M3, M4), and margarines enriched with beta-carotene, to compensate for the reduction of carotene during the oil decolorization process during refining. Esterification caused a significant decrease in the solid fat content (SFC) of initial fat blends and fatty acid profile analysis confirmed just less than 0.17-0.3% of trans fatty acid content (According to the definition of zero trans less than 0.5 g/12 g serving). Differential scanning calorimetry (DSC) measurement indicated that the interesterified samples possess lower melting points while showing binary or ternary crystallization peaks. The Polarized light microscopy (PLM) confirmed the presence of fine, desirable β´spherulite crystals, which are effective in creating the proper texture in margarine. Formulated margarines were evaluated and compared with one type of commercial margarine (as a control sample). According to the texture profile analysis (TPA) and organoleptic results, the M3 formula was chosen as the best formulation for margarine preparation (using Si-3 blending with the 35: 65 ratios of FHSO to SFO).

Keywords

Main Subjects

  • Norazlina, M. R., Jahurul, H. A., Hasmadi, M., Mansoor, A. H., Norliza, J., Patricia, M., Ramlah George, M. R., Noorakmar, A. W., Lee, J. S. and Fan, H. Y., “Trends in blending vegetable fats and oils for cocoa butter alternative application: A review”, Trends in Food Science & Technology, 116, 102 (2021).
  • Ribeiro, A. P. B., Basso, R. C., Grimaldi, R. and Gioielli, L. A., “Instrumental methods for the evaluation of interesterified fats”,Food Analytical Methods, 2 (4), 282 (2009).
  • Marangoni, A. G. and Garti, N., Edible oleogels: Structure and health implications, Elsevier, (2018).
  • Ribeiro, A. P. B., Basso, R. C., Grimaldi, R., Gioielli, L. A., dos Santos, A. O. and Cardoso, L. P., “Influence of chemical interesterification on thermal behavior, microstructure, polymorphism and crystallization properties of canola oil and fully hydrogenated cottonseed oil blends”, Food Research International, 42 (8), 1153 (2009).
  • Shahidi, F., Bailey's industrial oil and fat products, 1., John Wiley & Sons, New Jersey, (2005).
  • Warner, K. and Eskin, M., Methods to access quality and stability of oils and fat-containing foods, AOCS Publishing, (1995).
  • Omar, Z., Let, C. C., Seng, C. C. and Rashid, N. A., “Crystallisation and rheological properties of hydrogenated palm oil and palm oil blends in relation to crystal networking”, European Journal of Lipid Science and Technology, 107 (9), 634 (2005).
  • Rozendaal, A. and Macrae, A., “Interesterification of oils and fats”, in Lipid technologies and applications, Routledge, 223 (2018).
  • Kim, B. H., Lumor, S. E. and Akoh, C. C., “Trans-free margarines prepared with canola oil/palm stearin/palm kernel oil-based structured lipids”, Journal of Agricultural and Food Chemistry, 56 (17), 8195 (2008).
  • Zeitoun, M., Neff, W., List, G., Mounts, T., Neff, W., List, G. R. and Mounts T. L., “Physical properties of interesterified fat blends”, Journal of the American Oil Chemists' Society, 70 (5), 467 (1993).
  • D’Souza, V., DeMan, J. and DeMan, L., “Chemical and physical properties of the solid fats in commercial soft margarines”, Journal of the American Oil Chemists Society, 69 (12), 1198. (1992).
  • Berger, K., Jewell, G. and Pollitt, R., Oils and Fats”, In-Food microscopy, Vaughan, J. G. ed., Academic Press, London, (1979).
  • Oliveira, P. D., Rodrigues, A. M., Bezerra, C. V. and Silva, L. H., “Chemical interesterification of blends with palm stearin and patawa oil”, Food Chemistry, 215, 369 (2017).
  • Liu, Y., Meng, Z., Shan, L., Jin, Q. and Wang, X., “Preparation of specialty fats from beef tallow and canola oil by chemical interesterification: physico-chemical properties and bread applications of the products”, European Food Research and Technology, 230 (3), 457 (2010).
  • Zhou, X., Wang, W., Wang, C., Zhao, C., Peng, Q., Zhang, T. and Zhao, C., “Stability and in vitro digestibility of beta‐carotene in nanoemulsions fabricated with different carrier oils”, Food Science & Nutrition, 6 (8), 2537 (2018).
  • Zhang, X., Li, L., Xie, H., Liang, Z., Su, J., Liu, G. and Li, B., “Comparative analysis of thermal behavior, isothermal crystallization kinetics and polymorphism of palm oil fractions”, Molecules, 18 (1), 1036 (2013).
  • Shah, U., Patel, A. R., Van de Walle, D., Rajarethinem, P. S., Proctor, A. and Dewettinck, K., “CLA-rich soy oil margarine production and characterization”, Journal of the American Oil Chemists' Society, 91 (2), 309 (2014).
  • Pothiraj, C., Zuntilde, R., Simonin, H., Chevallier, S. and Le-Bail, A., “Methodology assessment on melting and texture properties of spread during ageing and impact of sample size on the representativeness of the results”, Journal of Stored Products and Postharvest Research, 3 (10), 137 (2012).
  • Danthine, S., Closset, S., Maes, J., Mascrez, S., Blecker, C., Purcaro, G. and Gibon, V., “Enzymatic interesterification to produce zero-trans and dialkylketones-free fats from rapeseed oil”, OCL, 29, 36 (2022).
  • Segura, N. and Jachmanián, I., “Zero-trans fats by enzymatic interesterification of blends beef tallow/rice bran oil”,OCL, 27 (2020).
  • Rousseau, D., Marangoni, A. G. and Jeffrey, K. R., “The influence of chemical interesterification on the physicochemical properties of complex fat systems. 2. Morphology and polymorphism”, Journal of the American Oil Chemists' Society, 75 (12), 1833 (1998).
  • Himawan, C., Starov, V. and Stapley, A., “Thermodynamic and kinetic aspects of fat crystallization”,Advances in colloid and interface science, 122 (1-3), 3 (2006).
  • Herrera, M., de Leon Gatti, M. and Hartel, R., “A kinetic analysis of crystallization of a milk fat model system”, Food Research International, 32 (4), 289 (1999).
  • Sahri, M. M. and Dian, N., “Formulation of trans-free and low saturated margarine”, Journal of Oil Palm Research, 23, 958 (2011).
  • Che Man, Y., Shamsi, K., Yusoff, M. and Jinap, S., “A study on the crystal structure of palm oil‐based whipping cream”, Journal of the American Oil Chemists' Society, 80 (5), 409 (2003).
  • Marikkar, J., Ghazali, H., Man, Y. C. and Lai, O., “Differential scanning calorimetric analysis for determination of some animal fats as adulterants in palm olein”, Journal of Food Lipids, 10 (1), 63 (2003).
  • Saadi, S., Ariffin, A. A., Ghazali, H. M., Miskandar, M. S., Boo, H. C., Abdulkarim, S. M., “Application of differential scanning calorimetry (DSC), HPLC and pNMR for interpretation primary crystallisation caused by combined low and high melting TAGs”, Food Chemistry, 132 (1), 603 (2012).
  • DeMan, L., DeMan, J. and Blackman, B., “Polymorphic behavior of some fully hydrogenated oils and their mixtures with liquid oil”, Journal of the American Oil Chemists’ Society, 66 (12), 1777 (1989).
  • Chawla, P., Deman, J. and Smith, A., “Crystal morphology of shortenings and margarines”, Food Structure, 9 (4), 2 (1990).
  • Hoerr, C., “Morphology of fats, oils, and shortenings”, Journal of the American Oil Chemists' Society, 37 (10), 539 (1960).
  • Tang, D. and Marangoni, A. G., “Microstructure and fractal analysis of fat crystal networks”, Journal of the American Oil Chemists' Society, 83 (5), 377 (2006).
  • Hassim, N. A. M., Kanagaratnam, S. and Dian, N. L. H. M., “Blended palm fractions as confectionery fats: A preliminary study”, Journal of Oil Palm Research,. 33 (2), 360 (2021).
  • Shi, Y., Liang, B. and Hartel, R. W., “Crystal morphology, microstructure, and textural properties of model lipid systems”, Journal of the American Oil Chemists' Society, 82 (6), 399 (2005).
  • Garti, N., Aronhime, J. and Sarig, S., “The rele of chain length and an emulsifier on the polymorphism of mixtures of triglycerides”, Journal of the American Oil Chemists' Society, 66 (8), 1085 (1989).
  • da Silva, R. C., Soares F. A. S. D. M., Maruyama, J. M., Dagostinho, N. R., Silva, Y. A., Ract, J. N. R. and Gioielli, L. A., “Microscopic approach of the crystallization of tripalmitin and tristearin by microscopy”, Chemistry and Physics of Lipids, 198, 1 (2016).
  • DeMan, L., D’Souza, V. and DeMan, J., “Polymorphic stability of some shortenings as influenced by the fatty acid and glyceride composition of the solid phase”, Journal of the American Oil Chemists Society, 69 (3), 246 (1992).
  • Sato, K. and Ueno, S., “Crystallization, transformation and microstructures of polymorphic fats in colloidal dispersion states”, Current Opinion in Colloid & Interface Science, 16 (5), 384 (2011).
  • Bayés-García, L., Calvet, T., Cuevas-Diarte, M. À., Ueno, S. and Sato, K., “Heterogeneous microstructures of spherulites of lipid mixtures characterized with synchrotron radiation microbeam X-ray diffraction”, Eng. Comm., 13 (22), 6694 (2011).
  • Tan, K., Lee, K., Mohamed, A. and Bhatia, S., “Palm oil: Addressing issues and towards sustainable development”, Renewable and Sustainable Energy Reviews, 13 (2), 420 (2009).
  • Metin, S. and Hartel, R. W., Crystallization of fats and oils, John Wiley & Sons, New York, p. 45 (2005).
  • Nguyen, V., Rimaux, T., Truong, V., Dewettinck, K. and Van Bockstaele, F., “Granular crystals in palm oil based shortening/margarine: A review”, Crystal Growth & Design, 20 (2), 1363 (2019).
  • Campioni, N. C., Pereyra, L. S., Ribeiro, A. P. B. and Alpuy, I. J., “Zero-trans fats designed by enzyme-catalyzed interesterification of rice bran oil and fully hydrogenated rice bran oil”, OCL, 28, 46. (2021).