Iranian Journal of Chemical Engineering (IJChE)
Scientific Journal

Isolation and Identification of a Bacterial Strain Producing Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from Municipal Landfill Soil

Document Type : Regular Article

Authors

Hanieh Karimnezhad
Farshad Rahimpour

Biotechnology Research Lab., Chemical Engineering Department, Faculty of Petroleum and Chemical Engineering, Razi University, Kermanshah 67149-67346, Iran

https://doi.org/10.22034/ijche.2026.566933.1582
Abstract
Conventional plastics have been a significant source of the environmental pollution, prompting considerable research into the development of biodegradable plastics using biological methods. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) has garnered particular attention due to its unique properties, including high flexibility and resistance to organic solvents. It has been demonstrated that certain microorganisms possess the intracellular capability to synthesize this biopolymer from organic waste. This study investigates bacteria that have been isolated from the municipal landfill of the Kermanshah Waste Recycling and Organic Fertilizer Production Company. Among the isolates, a strain capable of synthesizing biopolymers, exhibiting high similarity to the genus Stenotrophomonas geniculata strain Flmat 1, was identified via 16S rRNA gene sequencing. In order to verify the production of the biopolymer, Fourier-transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR) analyses were employed. The results have shown that the isolated bacteria is capable of producing PHBV from unrelated carbon sources from waste food and producing 2.835 g/L biopolymer with the yield of 0.52 g PHBV per gram of CDW using waste food, at the pH of 9, temperature of 33°C, concentrations of 13.25 g/L and 27.71 g/L of nitrogen and glucose respectively.

Keywords

Stenotrophomonas geniculata
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Food waste.

Subjects

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Iranian Journal of Chemical Engineering (IJChE)
Volume 22, Issue 4
Autumn 2025
Pages 43-55

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